Scanning Transmission Electron Tomography of Blood Platelets in Thick Sections

Nanomaterial datasets to advance tomography in scanning transmission electron microscopy

Electron tomography in materials science has flourished with the demand to characterize nanoscale materials in three dimensions (3D). Access to experimental data is vital for developing and validating reconstruction methods that improve resolution and reduce radiation dose requirements. This work presents five high-quality scanning transmission electron microscope (STEM) tomography datasets in ...

15. Scanning Transmission Electron Microscopy

Elemental mapping in scanning transmission electron microscopy

We discuss atomic resolution chemical mapping in scanning transmission electron microscopy (STEM) based on core-loss electron energy loss spectroscopy (EELS) and also on energy dispersive X-ray (EDX) imaging. Chemical mapping using EELS can yield counterintuitive results which, however, can be understood using first principles calculations. Experimental chemical maps based on EDX bear out the t...

Scanning electron microscopic observations on human blood platelets and their alterations induced by thrombin.

Since the introduction of transmission electron microscopy in hematology, blood platelets have frequently been the subjects of investigation using shadow cast technique or ultrathin sections on account of their unique native (circulating) form and their behavior (aggregation) and transformation during hemostasis (for review see; JOHNSON et al. 1961, DAVID-FERREIRA 1964, MARCUS and ZUCKER 1965, ...

Scanning transmission low-energy electron microscopy

We discuss an extension to the transmission mode of the cathode-lens-equipped scanning electron microscope, enabling operation down to the lowest energies of electrons. Penetration of electrons through free-standing ultrathin films is examined along the full energy scale, and the contribution of the secondary electrons (SEs), released near the bottom surface of the sample, is shown, enhancing t...